Review



rabbit α suv3  (Bethyl)


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    Structured Review

    Bethyl rabbit α suv3
    (A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, <t>SUV3,</t> and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.
    Rabbit α Suv3, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit α suv3/product/Bethyl
    Average 93 stars, based on 5 article reviews
    rabbit α suv3 - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "RNA triggers chronic stress during neuronal aging"

    Article Title: RNA triggers chronic stress during neuronal aging

    Journal: bioRxiv

    doi: 10.1101/2025.08.04.668575

    (A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, SUV3, and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.
    Figure Legend Snippet: (A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, SUV3, and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.

    Techniques Used: Software, Purification, Immunoprecipitation, RNA Sequencing, Derivative Assay, Sequencing, Reverse Transcription

    (A) Additional airyscan immunofluorescence-FISH images of the mitochondrial marker TOM70 (magenta) and MT-ND6 transcript (green) in Tdiff.1 neurons. Green arrowheads denote cytoplasmic MT-ND6 puncta; the white arrows denote the intensity profiles in the adjacent plot. Yellow Scale Bar = 2 μm. (B) Same as (A) but for the MT-RNR1 transcript in various transdifferentiated neuron lines (Tdiff.1/2/4). (C) Airyscan immunofluorescence images of the indicated mitochondrial proteins in Tdiff.1 neurons. Yellow Scale Bar = 2 μm. (D) Visualization of representative mitochondrial sequencing reads detected by PNPT1 eCLIP (n=2 replicates) in the indicated neuronal lines. The shaded region denotes the reverse-transcribed MT-ND6 gene. (E) Same as (D) but for SUV3 eCLIP.
    Figure Legend Snippet: (A) Additional airyscan immunofluorescence-FISH images of the mitochondrial marker TOM70 (magenta) and MT-ND6 transcript (green) in Tdiff.1 neurons. Green arrowheads denote cytoplasmic MT-ND6 puncta; the white arrows denote the intensity profiles in the adjacent plot. Yellow Scale Bar = 2 μm. (B) Same as (A) but for the MT-RNR1 transcript in various transdifferentiated neuron lines (Tdiff.1/2/4). (C) Airyscan immunofluorescence images of the indicated mitochondrial proteins in Tdiff.1 neurons. Yellow Scale Bar = 2 μm. (D) Visualization of representative mitochondrial sequencing reads detected by PNPT1 eCLIP (n=2 replicates) in the indicated neuronal lines. The shaded region denotes the reverse-transcribed MT-ND6 gene. (E) Same as (D) but for SUV3 eCLIP.

    Techniques Used: Immunofluorescence, Marker, Sequencing, Reverse Transcription



    Similar Products

    rabbit α suv3  (Bethyl)
    93
    Bethyl rabbit α suv3
    (A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, <t>SUV3,</t> and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.
    Rabbit α Suv3, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit α suv3/product/Bethyl
    Average 93 stars, based on 1 article reviews
    rabbit α suv3 - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, SUV3, and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.

    Journal: bioRxiv

    Article Title: RNA triggers chronic stress during neuronal aging

    doi: 10.1101/2025.08.04.668575

    Figure Lengend Snippet: (A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, SUV3, and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.

    Article Snippet: The following antibodies were used for eCLIP: 1 μg Rabbit α-PKR (Cell Signaling Technology #12297S); 10 μg Rabbit α-PNPT1 (Bethyl #A303-917A); and 2 μg Rabbit α-SUV3 (Bethyl #A303-056A).

    Techniques: Software, Purification, Immunoprecipitation, RNA Sequencing, Derivative Assay, Sequencing, Reverse Transcription

    (A) Additional airyscan immunofluorescence-FISH images of the mitochondrial marker TOM70 (magenta) and MT-ND6 transcript (green) in Tdiff.1 neurons. Green arrowheads denote cytoplasmic MT-ND6 puncta; the white arrows denote the intensity profiles in the adjacent plot. Yellow Scale Bar = 2 μm. (B) Same as (A) but for the MT-RNR1 transcript in various transdifferentiated neuron lines (Tdiff.1/2/4). (C) Airyscan immunofluorescence images of the indicated mitochondrial proteins in Tdiff.1 neurons. Yellow Scale Bar = 2 μm. (D) Visualization of representative mitochondrial sequencing reads detected by PNPT1 eCLIP (n=2 replicates) in the indicated neuronal lines. The shaded region denotes the reverse-transcribed MT-ND6 gene. (E) Same as (D) but for SUV3 eCLIP.

    Journal: bioRxiv

    Article Title: RNA triggers chronic stress during neuronal aging

    doi: 10.1101/2025.08.04.668575

    Figure Lengend Snippet: (A) Additional airyscan immunofluorescence-FISH images of the mitochondrial marker TOM70 (magenta) and MT-ND6 transcript (green) in Tdiff.1 neurons. Green arrowheads denote cytoplasmic MT-ND6 puncta; the white arrows denote the intensity profiles in the adjacent plot. Yellow Scale Bar = 2 μm. (B) Same as (A) but for the MT-RNR1 transcript in various transdifferentiated neuron lines (Tdiff.1/2/4). (C) Airyscan immunofluorescence images of the indicated mitochondrial proteins in Tdiff.1 neurons. Yellow Scale Bar = 2 μm. (D) Visualization of representative mitochondrial sequencing reads detected by PNPT1 eCLIP (n=2 replicates) in the indicated neuronal lines. The shaded region denotes the reverse-transcribed MT-ND6 gene. (E) Same as (D) but for SUV3 eCLIP.

    Article Snippet: The following antibodies were used for eCLIP: 1 μg Rabbit α-PKR (Cell Signaling Technology #12297S); 10 μg Rabbit α-PNPT1 (Bethyl #A303-917A); and 2 μg Rabbit α-SUV3 (Bethyl #A303-056A).

    Techniques: Immunofluorescence, Marker, Sequencing, Reverse Transcription